Specificity of anti WH7-BSA serum to WH7-BSA conjugate.

<p>Anti WH7-BSA serum was used at final dilution of 1∶5,000 and the strains tested were <i>E. faecalis</i> 12030 and <i>E. faecium</i> E1162. Inhibitor WH7-BSA conjugate at different concentrations 100, 20, 4 and 0.8 µg/mL was preincubated with anti-LTA antibodies for 1 hour at 4°C prior OPA. Opsonic killing of the target strain with non-absorbed antibodies was used to assess the reduction of opsonic killing produced by each inhibitor, asterisks denote statistical significance (** P<0.01, *** P value <0.001). The corresponding dilutions of inhibitor used in the OPA are indicated on the x-axis and the % killing on the y-axis. Bars represent the mean of data and the error bars represent the standard error of the mean.