Opsonophagocytic assay.

<p>This experiment was used to test the ability of antibodies raised against conjugates with WH5 and WH7, and BSA to mediate killing in the strain <i>S. aureus</i> MW2. Anti WH7-BSA sera mediate opsonophagocytic killing of <i>S. aureus</i> MW2 in vitro, while the sera anti-BSA and anti-WH5-BSA do not. Comparison of killing percentages of same dilutions (i.e. 1∶10 or 1∶50) with control anti BSA serum, only showed statistical significance for anti WH7-BSA (*** P value <0.001). The different sera and the corresponding dilutions used in the OPA are indicated on the x-axis and the % killing on the y-axis. Bars represent the mean of data and the error bars represent the standard error of the mean.