The downwards bending of the tail in <i>gpr22</i> morphants is rescued by the co-injection of <i>gpr22</i> mRNA.

<p>(<b>A–C</b>) Lateral views with anterior to the left of zebrafish embryos at 24 hpf injected at one cell stage with (<b>A</b>) water, (<b>B</b>) 0.2 mM fluorescein tagged <i>gpr22</i> MO, (<b>C</b>) 75 pg/nl <i>gpr22</i> mRNA or (<b>D</b>) 0.2 mM fluorescein tagged <i>gpr22</i> MO and 100 pg/nl <i>gpr22</i> mRNA. (<b>B, C</b>) Deregulation of <i>gpr22</i> results in a bending of the tail, a reduction in length of the anterior-posterior axis and heart edema <i>(arrow)</i>. (<b>E</b>) Quantification of the tail phenotype. (<b>D, E</b>) The curly tail of embryos injected with fluorescein tagged <i>gpr22</i> MO is rescued by the co-injection of <i>gpr22</i> mRNA in a dose-dependent manner. MO = morpholino, hpf = hours post-fertilization, n = number of analyzed embryos, WT = wild type.