The relationship between Tim-3 expression and the cytotoxicity of NK cells.

<p>(A) Representative flow cytometric dot plots of CD107a expression on NK cells from septic mice at 0, 4, 12 and 24 h after LPS injection were shown. (B) The bar graphs showed the percentage and MFI of CD107a expression on NK cells at different time points. (C) The bar graphs showed the percentage of CD107a⁺ cells between Tim-3⁺ and Tim-3⁻ NK cell subsets from septic mice at 24 h after LPS injection. (D, F) Representative flow cytometry histograms showed granzyme B and perforin expression in NK cells from normal and septic mice at 24 h after LPS injection. The MFI of granzyme B and perforin expression in NK cells was shown. (E, G) The MFI of granzyme B and perforin expression in NK cells was compared between Tim-3⁺ and Tim-3⁻ NK cell subsets. Data are mean ± SEM of at least three independent experiments. *<i>p</i><0.05, **<i>p</i><0.01, ***<i>p</i><0.001.