Keratinocyte-conditioned medium mediate proliferation and keratinocyte migration.

<p>Vitamin D- treated KCM from DFU were tested for cell migration induction. HaCaT cells were grown to confluence on 24-well tissue culture plates. Then each well was denuded by half from cells and re-coated with fibronectin, then we measured wound closure at 24, 48 and 72 hours post-incubation using as controls Epithelial Growth Factor (EGF), LL-37 or HBD-2, and the KCM with blocking antibodies either for HBD-2 (7A) or for LL-37 (7B). BrdU incorporation was used as an index of cellular proliferation (7C). Data are expressed as a percentage of the BrdU-positive cells (BrdU incorporation). Each value represents the median ± interquartile range. Values are the mean of 3 independent experiments. Asterisks show the treated groups with statistical difference when compared with KCM for each time point. * p<0.05, ** p<0.01.