Skeletal muscle overexpression of MCP1 induces local inflammation and alters insulin signaling and glucose metabolism <i>in vivo</i>.

<p>A) QPCR analyses of MCP1, CD68, CD11c, TNFα, IL1β, RANTES and CXCL1 expression were performed on quadriceps. C–D) <i>Ex-vivo</i> insulin signaling assay were performed on freshly isolated gastrocnemius. The histogram (D) represents the fold response to insulin on Akt phosphroylation in Wild-Type (WT) and MCK-MCP1-Tg mice. E) Genomic mitochondrial DNA was evaluated by qPCR in skeletal muscle of WT and transgenic mice. * stands for <i>P</i><0.05 and ** for <i>P</i><0.01; n = 6.