Effect of <i>Mcm7</i> RNAi on DNA replication, ployploidy and vitellogenesis.

<p>(A) <i>Mcm7</i> knockdown efficiency and <i>VgA</i> mRNA levels in the fat body of dsGFP control (iGFP), <i>Mcm7</i> RNAi (iMcm7), and iMcm7 further treated with methoprene for 48 h (iMcm7+JHA). **, <i>P</i><0.01; n.s, no significant difference; n = 4–6. (B) Comparison of fat body cell ploidy (a–f) and DNA synthesis (g–i) among iGFP, iMcm7 and iMcm7+JHA groups. Blue, nuclei; green, F-actin; red, <i>de novo</i> DNA synthesis. Enlarged images (4×) of a–c are shown in d–f, respectively. White bar, 20 µm; red bar, 5 µm. (C) Statistical analysis for the diameter of cell nuclei. *, <i>P</i><0.05; ***, <i>P</i><0.001; n = 80–100. (D) FACS analysis of DNA content in fat body cells after iMcm7 and iMcm7+JHA compared to iGFP. (E) Representative phenotypes of ovaries and primary oocytes after iMcm7 and iMcm7+JHA. (F) Statistical analysis for length*width index of primary oocytes. **, <i>P</i><0.01; ***, <i>P</i><0.001; n = 30.