Immunofluorescence showing expression of T2R1 in hPASMCs.

<p>hPASMCs were processed by standard immunofluorescence microscopy as described in the methods. T2R1 antisera was utilized to identify the indicated protein (first row). The negative control (second row) utilized an isotype-matched non-specific IgG as the primary antibody, and T2R38 antisera (third row) showed no signals. Rabbit anti-human T2R1 was visualized with goat anti-rabbit Alexa 488 antibody (green) and nuclei were stained with DAPI (blue). Merged images show that T2R1 localized partly on the cell surface of hPASMCs cells as indicated by arrows.