3D invasion of HuCCT1 cells into COL1 ECM over 6 days.

<p>(A) Phase-contrast image of protruding cells on day 3. (B) Summary data showing the total numbers of COL1-invading cells over 6 days. *<i>P</i><0.05 and *** p<0.001 compared with the control. Significance was analyzed by Student's <i>t</i>-test. Error bars, ± SEM. (C) Immunofluorescence image of HuCCT1 cell penetration (red arrowheads) into COL1 under control (top) and Dx1 (bottom) conditions. Scale bars = 100 µm.