Nrp1 expression does not contribute to the lack of effector function in tolerant CD8⁺ T cells.

<p>Naive WT (CD90.1⁺) and Nrp1-deficient (CD90.1⁺/90.2⁺) Gag-specific CD8⁺ T cells were transferred into Alb∶Gag recipients with (lower) or without (upper) a <i>Listeria</i> infection. Three days later, production of IFNγ and TNFα by transferred T cells was measured after overnight restimulation with Gag peptide. (A) Plots display T cell frequency (left) and IFNγ and TNFα production (right). Inset numbers are the percent of total splenocytes within the inscribed square region (left). Numbers in each quadrant represent the percent of gated CD8⁺ CD90.1⁺ T cells (right). (B) The percent of gated CD8⁺ CD90.1⁺ or CD8⁺ CD90.1⁺/90.2⁺ T cells that express IFNγ in differentially treated Alb∶Gag recipients was graphed, with each circle representing individual mice pooled from 4 separate experiments. Horizontal bars represent the average for each group (ns = not significant).