Apoptotic markers immunofluorescence assay.

<p>Photomicrographs of immunofluorescence analyses of kidney cytochrome c (green staining) of lean (a), <i>ob/ob</i> (b) and <i>ob/ob</i> treated with melatonin (c) mice. Immunofluorescence analyses of Bax (green staining) in kidney of lean (e), <i>ob/ob</i> (f) and <i>ob/ob</i> treated with melatonin (g) mice. Double immunofluorescence of caspase 3 (green staining) and caspase 9 (red staining) in kidney of lean (i), <i>ob/ob</i> (l) and <i>ob/ob</i> treated with melatonin (m) mice. Nuclei were stained with 4,6 diamidino-2-phenilindole. Bar equals 20 µm. The graphs summarize the quantitative analysis, respectively, of cytochrome c (d), Bax (h) and both caspase 3 and caspase 9 (n). *<i>p</i><0.05 <i>vs</i> lean and #<i>p</i><0.05 <i>vs ob/ob.</i>