Rapid cell proliferation of hiPSCs plated at low cell density on laminin-521 in Essential 8 medium.

<p>(A-I) Quantification of the number of 253G1, 201B7 and 409B2 cells expanded on laminin-521 in Essential 8 or mTeSR1 medium. Cell numbers were counted every 24 h after plating at 3.2×10⁴ cells/cm² (A, D, G), 1.6×10⁴ cells/cm² (B, E, H) and 8.0×10³ cells/cm² (C, F, I), respectively. Data are presented as the mean ± standard deviation (SD) of three independent experiments (*<i>P</i><0.05, **<i>P</i><0.01, *** <i>P</i><0.001, two-way repeated-measures ANOVA followed by a Bonferroni post-hoc test).