MSC immunophenotyping by flow cytometry.
- Louise Alessandra Mesentier-Louro (649707)
- Camila Zaverucha-do-Valle (649708)
- Almir Jordão da Silva-Junior (649709)
- Gabriel Nascimento-dos-Santos (649710)
- Fernanda Gubert (649711)
- Ana Beatriz Padilha de Figueirêdo (649712)
- Ana Luiza Torres (649713)
- Bruno D. Paredes (649714)
- Camila Teixeira (649715)
- Fernanda Tovar-Moll (491928)
- Rosalia Mendez-Otero (2222035)
- Marcelo F. Santiago (204865)
Publication date
October 2014
DOIAbstract
<p>MSC were analyzed from passages 3 to 5, after being frozen and thawed. (A) Forward Scatter (FSC) and Side Scatter (SC) profiles of MSC. The gate in (A) was drawn to exclude doublets of cells. Ninety-five percent of the analyzed cells were positive for CD90 and CD29 (B), 99.8% were negative for CD45 and CD11b-c (C), 99.8% were negative for CD34, and 99.6% were negative for CD45 (D). These results are consistent with the phenotype of multipotent mesenchymal stem cells.</p
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