Down-regulation of the αA, αB, β and γ-crystallins caused by the <i>miak</i> mutation of <i>Pitx3</i>.

<p>Confocal images show the double-labeled crystallin proteins (red) and DAPI (blue) in the lenses of wild-type and <i>miak</i>/<i>miak</i> mice at E12.5. Scale bar <i> = </i>100 µm. <b>A.</b> The αA-crystallins labeling of the lens. The expression patterns of αA-crystallins were similar in the lens epithelium (le) and lens fiber (lf) of the wild-type and <i>miak</i> mice; however, the αA-crystallins signals may be slightly reduced in the <i>miak</i> mice. <b>B.</b> The αB-crystallins labeling of the lens. The αB-crystallin signals were not detected in the <i>miak</i>/<i>miak</i>. <b>C.</b> The β-crystallin labeling of the lens. The β-crystallin signals were barely detected in the <i>miak</i>/<i>miak</i> lens. <b>D.</b> The γ-crystallin labeling of the lens. Immunohistochemistry reveals the dramatically reduced γ-crystallin signals in the <i>miak</i> mice as well as ectopic expression in the anterior region of the lens.