The phylogenetic tree for plant Na⁺/H⁺ antiporters was generated using MEGA4: Tree Explorer software.

<p>The evolutionary history was inferred using the neighbor-joining method and analyzed using bootstrap analysis with 500 replicates. Branches corresponding to partitions reproduced in less than 50% bootstrap replicates are collapsed. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using the Poisson correction method and are in the units of the number of amino acid substitutions per site. The GenBank Accession numbers for NHX proteins used are: VrNHX1 (AEO50758.1), VuNHX1 (AEO72079.2), GmNHX1 (AAY430061.1), CkNHX1 (ABG89337.1), MsNHX1 (AAS84487.1), CaNHX1 (ADL28385.1), TrNHX1 (ABV00895.1), LtNHX1 (ACE78322.1), AhNHX1 (ADK74832.1), AtNHX1 (NM_122597.2).