In A1 cells the different kinetics of p-ERK1/2 activation are abolished by β-cyclodextrin administration.

<p>Western blot detection of p-ERK1/2 and ERK1/2 proteins in proliferating (A) and non-proliferating A1 cells (C) treated at indicated time with E₂ and ß-cyclodextrin. Two specific bands were observed respectively at 44 and 42 kDa. Each blot is representative of three separate experiments. The diagrams show the relative quantization of p-ERK1/2 and ERK1/2 in proliferating (B) and non-proliferating (D) A1 cells. Data are expressed as ratios of p-ERK1-2/ERK1-2. The trend line shows the kinetic of p-ERK1/2 following E₂ stimulation. Asterisks represent p<0.01 when compared to control cultures treated with vehicle (ANOVA, Scheffè F-test).