The effect of Areg on PKC δ- and θ-mediated FSH-induced meiotic resumption in COCs.

<p>The germinal vesicle breakdown (GVB) ratio of COCs cultured in FSH-supplemented media with or without the PKC δ and θ inhibitor rottlerin (MTX) (<b>A</b>), PKC ζ pseudosubstrate inhibitor (PI) (<b>B</b>), or PKC α and βI inhibitor Gö6976 (<b>C</b>) for 22–24 h. The GVB ratio of COCs cultured in media supplemented with either 0.1 µM MTX, 0.05 IU/mL FSH, MTX plus FSH, or MTX plus FSH plus 100 ng/mL Areg for 22–24 h (<b>D</b>). After COCs were cultured in media with either FSH (0.05 IU/mL) and/or 0.1 µM MTX for 4 h, the mRNA levels of <i>Areg</i>, <i>Ereg</i> and <i>Btc</i> in cumulus cells were detected using real-time PCR (<b>E</b>). Letters (a–d) on different columns in each chart indicate significant difference between groups (<i>p</i><0.05). AR: Areg.