Overlap between predicted CD8 and CD4 epitopes in Env-V2 and -V3 in sequences from HIV-1-infected RV144 participants.

<p>The x-axis corresponds to the V2 and V3 sequence, and the y-axis corresponds to the number of predicted epitopes starting at each position. The epitope predictions correspond to all unique HLA/peptide combinations, hence the number of epitope predictions starting at a specific location can surpass the number of subjects in the cohort because a given peptide can be predicted as an epitope for multiple HLA alleles. The amino acids in red correspond to sites that were identified as genetic signatures that distinguished breakthrough sequences from vaccine and placebo recipients.