Biochemical analysis after 47 days of culture.

<p>(<b>a</b>) Cell metabolic activity [activity/total DNA]. (<b>b</b>) DNA content per scaffold [ng/mL]. (<b>c</b>) Calcium content per scaffold [mg/mL]. (<b>d</b>) ALP activity normalised to cell number [µg/p-nitrophenol/total DNA]. Data is shown as average ± standard deviation (<i>n</i> = 5). Asterisks indicate significant (<i>p</i><0.05) or highly significant (<i>p</i><0.01) difference between the groups. Abbreviations: Sp.O.W: spinner flask culture in osteogenic medium with weekly x-ray; Sp.C.W: spinner flask culture in control medium with weekly x-ray; Sp.O.E: spinner flask culture in osteogenic medium with x-ray at the end; St.O.E: static culture in osteogenic medium with x-ray at the end; St.C.E: static culture in control medium with x-ray at the end.