Gene expression analysis.

<p>Images showing normalised fold expression against <i>GAPDH</i> for the genes <i>ALP</i> (<b>a</b>), <i>DSPP</i> (<b>b</b>), <i>Nestin</i> (<b>c</b>), <i>Collagen type I</i> (<b>d</b>) and <i>Osteocalcin</i> (<b>e</b>). Data is shown as average ± standard deviation of three samples. Asterisks indicate significant (<i>p</i><0.05) or highly significant (<i>p</i><0.01) difference between the groups. Abbreviations: Sp.O.W: spinner flask culture in osteogenic medium with weekly x-ray; Sp.C.W: spinner flask culture in control medium with weekly x-ray; Sp.O.E: spinner flask culture in osteogenic medium with x-ray at the end; St.O.E: static culture in osteogenic medium with x-ray at the end; St.C.E: static culture in control medium with x-ray at the end.