Binding affinities of scFv antibodies.

<p>The <i>K</i>_D of purified scFv antibody was measured on yeast displayed antigen domains and compared with the <i>K</i>_D for binding to antigen (Ag) expressing mammalian cells by flow cytometry. scFv mAbs were incubated with the yeast cells transformed and induced for antigen expression, or with the antigen overexpressing cancer cells; SKOV3 cells for HER2 mAbs, MDA-MB-231 for EphA2 and CD44 mAbs, Colo205 for EphB3 mAbs.