<p>Binding of fifteen polyclonal phage Ab outputs (A) and nine phagemid Ab outputs (B) from the first, second and third round of selection compared to binding to an irrelevant yeast displayed antigen. The antigen used for selection is shown above each dot-plot.</p
Yeast surface display is a proven tool for the selection and evolution of ligands with novel binding...
<p>Phage clones binding to CHO-K1/VPAC1 cells (<i>blue bars</i>) and wild-type CHO-K1 cells (<i>red ...
The combinatorial phage display library approach to antibody repertoire cloning offers a powerful to...
<p>Antibodies are first selected against Ag85 using two rounds of phage display, after which the who...
<p>A) The density of each protein domain on the induced yeast surface was quantitated and plotted ag...
3non vitro display technologies have put together the generation of large antibody libraries with se...
<p>2A shows the yeast population after cloning the Ag85 selected phage display output. The upper rig...
(A) Three rounds of bio-panning were performed with positive samples, in which M2 antigens were disp...
<p>N.D. This test was not done.</p><p>Antigen and antigen domain used for selection are indicated al...
<p>Flow cytometry analysis of the fraction of target-binding clones in the enriched population after...
<p>Two selection methods are available to screen affinity-altered proteins. One is to use the <i>GFP...
<p>(<b>A</b>) anti-cMet-B10, (<b>B</b>) adalimumab (<b>C</b>) matuzumab and the respective controls ...
<p>a) The phage library, containing billions of different phages, was amplified from the bacterial s...
<p>(A) A schematic diagram of phage panning against antigens expressed in yeast display system <a hr...
Phage display libraries represent a relatively easy way to generate binding ligands against a vast n...
Yeast surface display is a proven tool for the selection and evolution of ligands with novel binding...
<p>Phage clones binding to CHO-K1/VPAC1 cells (<i>blue bars</i>) and wild-type CHO-K1 cells (<i>red ...
The combinatorial phage display library approach to antibody repertoire cloning offers a powerful to...
<p>Antibodies are first selected against Ag85 using two rounds of phage display, after which the who...
<p>A) The density of each protein domain on the induced yeast surface was quantitated and plotted ag...
3non vitro display technologies have put together the generation of large antibody libraries with se...
<p>2A shows the yeast population after cloning the Ag85 selected phage display output. The upper rig...
(A) Three rounds of bio-panning were performed with positive samples, in which M2 antigens were disp...
<p>N.D. This test was not done.</p><p>Antigen and antigen domain used for selection are indicated al...
<p>Flow cytometry analysis of the fraction of target-binding clones in the enriched population after...
<p>Two selection methods are available to screen affinity-altered proteins. One is to use the <i>GFP...
<p>(<b>A</b>) anti-cMet-B10, (<b>B</b>) adalimumab (<b>C</b>) matuzumab and the respective controls ...
<p>a) The phage library, containing billions of different phages, was amplified from the bacterial s...
<p>(A) A schematic diagram of phage panning against antigens expressed in yeast display system <a hr...
Phage display libraries represent a relatively easy way to generate binding ligands against a vast n...
Yeast surface display is a proven tool for the selection and evolution of ligands with novel binding...
<p>Phage clones binding to CHO-K1/VPAC1 cells (<i>blue bars</i>) and wild-type CHO-K1 cells (<i>red ...
The combinatorial phage display library approach to antibody repertoire cloning offers a powerful to...