Acylated LprG binds lipoglycans in Mtb.

<p>SDS-PAGE analysis of proteins and co-purifying molecules isolated from Mtb H37Ra, <i>M. smegmatis</i> or <i>E. coli</i>. (<b>A</b>) Western blot with monoclonal anti-hexahistidine (anti-His₆) to detect LprG or LprA. Mycobacterial components associated with lipoproteins were detected using (<b>B</b>) monoclonal anti-LAM antibody CS-35 or (<b>C and D</b>) rabbit polyclonal anti-Mtb antibody that detects both LAM and LM. Blots are representative of at least three independent experiments.