Neutralization effect of an anti-heat shock protein 70 monoclonal antibody on the natural killer cell phenotype.

<p>Mice received intraperitoneal injections of an anti-heat shock protein (HSP) 70 monoclonal antibody or isotype-matched mouse immunoglobulin G (6 mice per group) just before fasting. After a 3-day fast, liver lymphocytes were harvested for phenotyping by flow cytometry. (A) The distribution of TRAIL and CD69 expression on electronically gated TCRβ⁻ NK1.1⁺ natural killer (NK) cells is indicated with solid lines (shaded areas). The percentage and mean fluorescence intensity (MFI) of TRAIL⁺ or CD69⁺ NK cells are provided. The dotted lines represent the distribution in the negative control. (B) TCRβ⁻ NK1.1⁺, (C) TRAIL⁺ and CD69⁺ NK cell proportions, and (D) MFI from NK cells positive for those markers are shown in bar graphs as mean plus standard deviation (n = 6). The difference among the groups was analyzed using the independent samples T test; *<i>p</i> <0.05.