Cellular localization of <i>Prss56 and Glul</i> in B6 (C57BL/6J) and <i>Mfrp^rd6/Mfrp^rd6</i> mice.

<p>(A) By <i>in situ</i> hybridization, in B6 (C57BL/6J) controls at P14, we observed <i>Prss56</i> transcript in only very few cells of the inner nuclear layer (INL) of the retina (top panel), whereas in <i>Mfrp^rd6/Mfrp^rd6</i> mutants, an intense staining of <i>Prss56</i> transcript was observed in INL of the retina (bottom panel). (B) By 2-plex <i>in situ</i> hybridization, in B6 controls, we observed co-localization of <i>Prss56</i> (red) and <i>Glul</i> (pseudo colored green) transcripts in only a few cell body of the (INL) of the retina (top panel), whereas in <i>Mfrp^rd6/Mfrp^rd6</i>, strong co-localization of <i>Prss56</i> and <i>Glul</i> transcripts in the cell body of the INL of the retina was observed (bottom panel). (C) Glutamine synthetase (GS) staining of Müller cells. In both C57BL/6J (B6) and <i>Mfrp^rd6/Mfrp^rd6</i> mice, Müller cells marked with glutamine synthetase showed a similar localization pattern (inset, top and bottom panels) as observed for <i>Prss56 in situ</i> hybridization staining, suggesting that Müller cells in the INL of retina express <i>Prss56</i> transcripts at P14.