Schedule of immunization and anti-Tat antibody responses in cynomolgus monkeys vaccinated with Tat/H1D and Tat/Alum.

<p>(<b>A</b>) Nine monkeys were injected intramuscularly with Tat/H1D microspheres at weeks 0, 4, 12 and 18, and boosted subcutaneously with the Tat in Alum at weeks 21 and 36, respectively. Nine control monkeys were primed with H1D alone boosted with Alum alone. (<b>B</b>) IgM and (<b>C</b>) IgG antibody titers in vaccinated monkeys. The arrows on the top of the each panel indicate the time at which the Tat/H1D, the Tat/Alum or H1D and Alum alone were given. The dashed lines indicate the cut-off values (samples showing titers <1∶25 for IgM and <1∶100 for IgG were scored as negative).