Phenotypic analysis of <i>C. dubliniensis tloΔ</i> and <i>med3</i>Δ mutants.

<p>The rate of formation of true hyphae in water supplemented with 10% serum was measured in the <i>tlo1</i>Δ, <i>tlo2</i>Δ and <i>tlo1</i>Δ/<i>tlo2</i>Δ (<i>tlo</i>ΔΔ) mutants relative to Wü284 (A), in the <i>TLO1</i> and <i>TLO2</i> reintegrants (<i>tlo</i>ΔΔ+<i>TLO1/2</i>)(B) and in the <i>med3</i>Δ mutant (C). Measurements were taken over a period of 5 h by counting the number of cells producing unconstricted germ-tubes and expressing this value as a percentage of the whole population. (D) Cellular morphology of Wü284, the <i>tlo1</i>Δ/<i>tlo2</i>Δ (<i>tlo</i>ΔΔ) mutant and <i>TLO1</i> and <i>TLO2</i> reintegrated strains in 10% serum following staining with calcofluor white. (E) Oxidative stress susceptibility in <i>tlo</i>Δ mutants. Susceptibility to oxidative stress was assessed by spotting 20 µl aliquots of 10-fold serially diluted suspensions (10⁶ to 10³ cells/ml) of the indicated strains on YEPD agar plates containing 7 mM H₂O₂ or 100 mM menadione. Plates were incubated for 48 h at 37°C.