Ensemble steady state acceptor fluorescence anisotropy values of MDA-MB 231 cells expressing different Cdc42 constructs.

<p>MDA-MB 231 cells were expressing different Cdc42 constructs and imaged live at 37°C, excited respectively in epifluorescence and in TIRF excitation. Measurements were compared using either two-tailed paired <i>t</i>-test (A) or two-tailed unpaired <i>t</i>-test with 95% confidence intervals (B, C) (***<i>p</i><0.001,**<i>p</i><0.01, *<i>p</i><0.05, ns non significant).