siRNATSC1 and siRNATSC2 induce opposite effects on NIH 3T3 fibroblast migration.

<p>Cells were transfected with siRNA TSC1 (A), siRNA TSC2 (B), or control siRNA. 48 h post-transfection, protein levels were detected by immunoblot analysis with anti-TSC1 or anti-TSC2 antibodies. C, Upper panel: Representative image of hemacolor-stained membrane with migrated NIH 3T3 fibroblasts for 4 h. 3T3 fibroblasts were transfected with siRNA TSC1, siRNA TSC2, and siGLO RISC-Free siRNA as control cells, serum-deprived followed by migration assay in the presence or absence of 10 ng/ml PDGF performed in triplicate for each experimental condition. C, Lower panel: Statistical analysis of NIH 3T3 cell migration. Data represent mean values ± SE from two independent experiments, six repetitions in each experiment. *P<0.01 for siRNA TSC1 vs. control siRNA, **P<0.001 for siRNA TSC2 vs. control siRNA by ANOVA (Bonferroni-Dunn).