Determining fatty acid oxidation.

<p>A. Schematic illustration of biochemical pathway of fatty acid oxidation. B. Kinetic OCR response of SF188f cells to palmitate (150 µM). C. Kinetic OCR response in SF188f cells to octanoate (1 mM). SF188f cells were plated at 15,000 cells/well in XF96 V3 cell culture plates 24–28 hours prior to the assays. Assay medium was the substrate-free base medium supplemented with 5.5 mM glucose and 50 µM carnitine. The OCR value was not normalized. A representative experiment out of four is shown here. Each data point represents mean ± SD, n = 6.