Other membrane-active substances, which are structurally unrelated to bile acids also activate BASIC.

<p>A–C) Representative current traces (black) of BASIC-expressing oocytes showing that the application of 400 µM SDS (A, left panel), 500 µM N-Lauroylsarcosine (NL) (B) and 100 µM Triton-X 100 (C) increased the activity of BASIC. The application of 100 µM diminazene (DIMI) alone or together with SDS (A, right panel), NL (B) or Triton-X 100 (C) inhibited BASIC currents. Non-injected oocytes did not respond to the application of the substances (grey traces). Dotted lines represent the 0 current level. (D) Quantitative comparison of current amplitudes induced by SDS, NL or Triton-X 100. Currents were normalized to the DIMI-sensitive current in the absence of stimulatory substances, which had an amplitude of 0.25±0.03 µA (<i>n</i> = 8). Error bars represent SEM. Statistical significances were tested using one-way ANOVA followed by Tukey's multiple comparison test versus control, **p<0.01, ***p<0.001, <i>n</i> = 8. (E) Representative current traces of oocytes expressing ASIC1a or HyNaC2/3/5. Application of pH 5 or 5 µM RFamide I, respectively, activated the channels, while application of 400 µM SDS, 500 µM NL or 100 µM Triton-X 100 did not.