Aβ25–35-induced growth inhibition of SH-SY5Y cells is mediated by autophagy.

<p>(A) Annexin V/PI staining of apoptosis of SH-SY5Y cells treated with different doses of Aβ_25–35 for 24 h. (B) Hoechst staining of apoptosis of SH-SY5Y cells treated with different doses of Aβ_25–35 for 24 h. (C) Immunoblotting of Beclin 1 and LC3 expression with SH-SY5Y cell lysates. Cells were treated with 10 µM Aβ_25–35 for additional 24 h after transfection with random siRNA or beclin 1 siRNA for 48 h. (D) MTT assay of cell viability of SH-SY5Y cells treated with 10 µM Aβ_25–35 for 24 h after transfection with random siRNA or beclin 1 siRNA for 48 h.