Westeron blot and qRT-PCR detetion of TGF-β1 in the middle ear mucosa.

<p>Expression changes of TGF-β1 in protein level are obviously observed in all groups of guinea pig (A) and rat (B). (C) In guinea pig group, statistic analysis reveals that TGF-β1 is increased in TS group, decreased in captopril and losartan treated group but still higher than that of the control group. The same tendency also can be seen in rats group (D). (E) Expression of TGF-β1 in RNA level increases in TS group, and decreases in captopril and losartan treated group at the end of week 6. Each bar represents the mean percent ± SEM, n = 19 (each of the three groups). *<i>p</i><0.05, **<i>p</i><0.01 as conducted.