Deletion of <i>atfA</i> does not affect chitin deposition and cell wall integrity.

<p>(A) <i>P. marneffei</i> wild type (F4), <i>atfA</i> mutant (SC) and <i>atfA</i> complemented (AC1) strains were grown for seven day at 25°C on PDA and stained with CFW day four and day seven to visualize cell wall and septa. Scale bar represents five micrometers. (B to M) five microliters of cell dilutions (5×10⁴ to 5 cells) of wild type, <i>atfA</i> mutant and <i>atfA</i> complemented strains were inoculated on media supplemented with 5 µg/ml CFW(C and I), 0.004% SDS (D and J), 10 µg/ml and 4 µg/ml amphotericin B (F and L) and 8 µg/ml and 0.8 µg/ml itraconazole (G and M). (B and E) and (H and K) represent MM control plates at 25°C and 37°C, respectively.