BG01V/hOG hEBs (formed under -ROCK/-spin condition) differentiated into tissue lineages specific to each of the three embryonic germ layers.

<p>(<b>a</b>) RT-PCR analysis demonstrated gene expression profiles that are specific to representative lineages associated with each germ layer, i.e., neural (ectoderm), pancreatic (endoderm), and cardiac (mesoderm) lineages. (<b>b</b>) At 25 days when treated with the cardiac differentiation protocol, western blot confirmed expressions of cardiac α-actin and cardiac troponin-C proteins that are representative of cardiac lineage.