Polysome gradient analyses of <i>dhh1</i> mutant cells grown in lactic or formic acids.

<p>A: Absorbance profiles along a polysomes gradient obtained with cells grown in lactic acid. The fractions corresponding to the polysomes are indicated. B: the polysomes fractions obtained in acetic and lactic acid were pooled and the percentage of <i>JEN1</i> mRNA contained in this fractions compared to the rest of the gradient was quantified by RT-QPCR. In contrast to previous Q-PCR experiments, SCR1 could not be used as a reference because it is not translated. Hence, <i>ACT1</i> was used as a control for an mRNA which was actively transcribed in both lactic and formic acids. The experiments were performed three times on biologically independent samples. The difference of <i>JEN1</i> abundance in polysomal fractions between lactic and formic acid was not significant, according to a Student test.