a<p>The results were obtained dividing the sum of the copy number from a total of eight replicates (two 0.5 µg replicates in the 1<sup>st</sup> qPCR and six 0.5 µg replicates in the 2<sup>nd</sup> qPCR) by 4 and expressed as HIV DNA copy number/µg of DNA.</p>b<p>Taking into account the WBC number, the results can be reported as copies/ml of blood with the formula: (copies/µg DNA)×WBC no. per ml/142857 cells, assuming that 142857 cells are present in one µg of DNA. WBC counts/µl are 3980 (sample 1), 7480 (sample 2), 8660 (sample 3), 5610 (sample 4), and 6620 (sample 5).</p>c<p>Taking into account %CD4+, the results can be reported as copies/10<sup>4</sup> CD4+ with the formula: [(copies/µg DNA)/(CD4+/WBC×142857 WBC)]×10<sup>4</sup>. The %CD4...
This thesis is concerned with the development of the polymerase chain reaction (PCR) as an accurate ...
Abstract Among the different markers of HIV persistence in infected cells, total HIV DNA is to date ...
Copyright © 2007, American Society for Microbiology. All Rights Reserved.We have adapted our establi...
<p><b>A)</b> Total <i>pol</i> HIV copies were quantified by ddPCR within Vδ2 cells (n = 12), total r...
<p>The bars represents the percentage of HIV-1 DNA copies and p24 values (normalized per 10<sup>6</s...
In the nucleus of HIV-1 infected cells, unintegrated HIV-1 DNA molecules exist in the form of one an...
In the nucleus of HIV-1 infected cells, unintegrated HIV-1 DNA molecules exist in the form of one an...
Total HIV-2 DNA quantification in CD3-CD4-CD14+ Monocytes, CD25+CD69+HLADR+ activated CD4 T-cells an...
A novel colorimetric assay was developed and validated for accurate quantitation of HIV DNA in PBMCs...
<p>(A) HIV DNA copy numbers in PBMC-derived, purified CD4+ T cells at start of STI (STI-w0), and 2 (...
A novel colorimetric assay was developed and validated for accurate quantitation of human immunodefi...
Deoxyribonucleic acid (DNA) of the human immunodeficiency virus (HIV) provides the most sensitive me...
International audienceAbstract Total HIV DNA is a standard marker to monitor the HIV reservoir in pe...
Background: The quantitative measurement of various HIV-1 DNA forms including total, unintegrated an...
<div><p>Deoxyribonucleic acid (DNA) of the human immunodeficiency virus (HIV) provides the most sens...
This thesis is concerned with the development of the polymerase chain reaction (PCR) as an accurate ...
Abstract Among the different markers of HIV persistence in infected cells, total HIV DNA is to date ...
Copyright © 2007, American Society for Microbiology. All Rights Reserved.We have adapted our establi...
<p><b>A)</b> Total <i>pol</i> HIV copies were quantified by ddPCR within Vδ2 cells (n = 12), total r...
<p>The bars represents the percentage of HIV-1 DNA copies and p24 values (normalized per 10<sup>6</s...
In the nucleus of HIV-1 infected cells, unintegrated HIV-1 DNA molecules exist in the form of one an...
In the nucleus of HIV-1 infected cells, unintegrated HIV-1 DNA molecules exist in the form of one an...
Total HIV-2 DNA quantification in CD3-CD4-CD14+ Monocytes, CD25+CD69+HLADR+ activated CD4 T-cells an...
A novel colorimetric assay was developed and validated for accurate quantitation of HIV DNA in PBMCs...
<p>(A) HIV DNA copy numbers in PBMC-derived, purified CD4+ T cells at start of STI (STI-w0), and 2 (...
A novel colorimetric assay was developed and validated for accurate quantitation of human immunodefi...
Deoxyribonucleic acid (DNA) of the human immunodeficiency virus (HIV) provides the most sensitive me...
International audienceAbstract Total HIV DNA is a standard marker to monitor the HIV reservoir in pe...
Background: The quantitative measurement of various HIV-1 DNA forms including total, unintegrated an...
<div><p>Deoxyribonucleic acid (DNA) of the human immunodeficiency virus (HIV) provides the most sens...
This thesis is concerned with the development of the polymerase chain reaction (PCR) as an accurate ...
Abstract Among the different markers of HIV persistence in infected cells, total HIV DNA is to date ...
Copyright © 2007, American Society for Microbiology. All Rights Reserved.We have adapted our establi...