SRL induced apoptosis involves alteration of regulators.

<p>(<b>A</b>) Immunohistochemistry of MCF-7 cells after treatment with SRL. MCF-7 cells were grown on cover slips and incubated with 20 µg/ml SRL for 36 h followed by staining with FITC-SRL, Anti-active caspase-3 primary antibody followed by pycoerythrin labelled secondary antibody and images were observed by fluorescence microscopy. (<b>B</b>) SRL-induced apoptosis in MCF-7 cells involves alteration of regulators in both the extrinsic and intrinsic apoptosis pathways. MCF-7 cells were incubated with SRL (20 µg/ml) for different time intervals. At specific time intervals whole cell protein was obtained, separated by electrophoresis, blotted on to Immobilon polyvinylidene difluoride membrane and probed with antibodies against FasL, FADD, tBID, PARP, pro-caspase-8, -9,-3 or active caspase-8, -9,-3. The bands were visualized using chemiluminescence kit. The blots were stripped and reprobed for β-actin which was used as loading control.