Accumulation of virus-derived small interfering RNAs (vsiRNAs) of plus polarity in WSMV-, TriMV- or WSMV plus TriMV-infected wheat cultivars Arapahoe and Mace at 18°C and 27°C.

<p>WSMV and TriMV minus-strand RNA-specific DIG-labeled riboprobes complementary to nucleotides 9384 to 6303 and 10,266 to 6401, respectively, were used for Northern blot hybridization. Riboprobes hydrolyzed to ∼50 nt were used for Northern hybridization of small RNAs. Ethidium bromide stained TBE-urea polyacrylamide gels showing low molecular weight (LMW) RNAs as sample loading control are presented under the Northern blots. Ar: Wheat cv. Arapahoe; Ma: Wheat cv. Mace; WS: WSMV; Tri: TriMV; Bu: buffer.