ScFvE3 preferentially binds to RhoB active conformation.

<p>A, Binding affinities of two selected scFvs (A5 and E3) for 6xHis-RhoAL63 (AL63), 6xHis-RhoBL63 (BL63) were measured by competitive ELISA on dilution of 6his-RhoL63 displayed in molar logarithmic scale (Log M), as described in experimental procedures. K_d values were determined by nonlinear regression and listed in the insert table (mean ± SD, n = 3 each). B, The specificity of the purified scFvE3 for the active form of the recombinant wild type GST-RhoB loaded with either GDP or GTPγS was assessed by ELISA. Results are expressed as absorbance at 450 nm (mean ± SD, Mann-Whitney test, n = 4).