Primer sequences for site directed-mutagenesis.

<p>Couples primers containing the mutations are designed <i>in silico</i> using the PrimerX software (<a href="http://www.bioinformatics.org/primerx/" target="_blank">http://www.bioinformatics.org/primerx/</a>) following the manufacturer's recommendations. The primers were synthesized <i>in vitro</i> and purified by HPSF (High Purity Salt Free or Highly purified salt-free, Eurofins MWG Operon).</p><p>Primer sequences for site directed-mutagenesis.