Stat92E transcriptional reporters are expressed in glia and transiently activated after axotomy.

<p>(A–D) Single slice confocal images; (A) 10XStat92E-GFP reporter expression in adult brain in uninjured control animals, one day after maxillary palp, and one day after antennal ablation. Glial nuclei are labeled with α-Repo (blue), glial membranes with α-Draper (red), and Stat92E transcriptional reporter activity with α-GFP (green). (B) 10XStat92E-dGFP in control (10xStat92E-dGFP/+;<i>repo-gal4</i>/+), and <i>stat92E^RNAi</i> (10xStat92E-dGFP/<i>stat92e</i>^RNAi;<i>repo-gal4</i>/+) backgrounds, uninjured and one day after antennal ablation. (C) High magnification image showing colocalization of Repo⁺ glial nuclei (arrows) and 10XStat92E-dGFP reporter activity in cortex glial cells following injury. Asterisks mark antennal lobe region. (D) Colocalization of mCherry driven by <i>dee7-Gal4</i> with the 10xStat92E-dGFP reporter activity one and three days after antennal ablation.