Enforced miR-509 resulted in inhibition of growth of 3 B-ALL cell lines, NALM6, REH and RCH-ACV.

<p>(A) Viable cell numbers measured via trypan blue dye exclusion counts of NALM6 cells transduced with either miR-509 lentivirus or empty vector (EV#1); 25,000 cells were plated for each sample starting at 3 days after transduction. (B) AlamarBlue cell growth assay on day 7 after transduction of NALM6 cells transduced with either miR-509 lentivirus or EV#1. Values for miR-509 were normalized to EV#1. (C) Viable cell counts of RCH-ACV cells based on trypan blue exclusion counts, initial plating of 25,000 cells for both samples on 3 days after transduction. Means ± SEMs are plotted, and SEMs for miR-509 were very small. (D) Cell growth of RCH-ACV transduced with either EV#1 or miR-509 overexpressing lentivirus using alamarBlue cell growth assay conducted on day 7 after transduction. Values for miR-509 were normalized to EV#1. (E) MiR-509-transduced REH cells reduced growth compared to EV#1 in an alamarBlue cell growth assay. Cells were transduced 7 days prior to addition of alamarBlue. (A to E) Means ± SEMs, 3 independent experiments done in triplicates. Statistical analysis was done by Student's <i>t</i> test. *p<0.05, **p<0.01.