Single-molecule stretching experiments on MvaU-DNA.

<p>(A) In the presence of MvaU, the DNA was stiffened as indicated by higher extension compared to naked DNA at the same force. Along with DNA stiffening, increase in DNA folding at higher concentration was also observed, indicated by either hysteresis or DNA folding. The DNA folding at 600 nM MvaU occurred at higher force, and the force-increase scan was not recorded to prevent the DNA extension from dropping below the minimal measurable extension. (B) The time-course data of the stretching experiment in panel A, showing the dynamics of force-decrease that leads to progressive DNA folding, followed by force-increase that gradually unfold the MvaU-DNA.