Pipeline of the exome-seq data analysis workflow.

<p>After processing the data using the GATK pipeline, this filtering workflow was derived to identify SNPs which were associated with measures of susceptibility across the racial and etiology groups of cases. SNPs were filtered based on strength of association, coding effect, and functional prediction prior to testing for association with other ARDS phenotypes. *, The sample contains African American and Caucasian patients, so the EUR and ASW healthy controls from 1000 Genomes were used for comparison; **, In the 1000 Genomes Project exome sequence, the same 714,074 SNPs are present for all 440 EUR and ASW; §, HWE = Hardy Weinberg Equilibrium, p>0.0001; +, African American with pneumonia, African American with sepsis, Caucasian with pneumonia, Caucasian with sepsis; + +, χ² test of ARDS vs. respective 1000 Genomes Project control groups; ‡, SNPs with P-value <0.01 in the overall comparison, Caucasian ARDS comparison, and African American comparison with 1000 Genomes were filtered further by p<0.01 in the sepsis comparison and pneumonia comparison; ‡ ‡, All ARDS cases, all pneumonia cases, all sepsis cases, all African American cases, all Caucasian cases.