NLRP3/caspase-1 inflammasome is the major source of IL-1β for parasite control in mφs.

<p>THP-1 mφs were incubated with <i>T. cruzi</i> in the presence or absence of cycloheximide (CHX,), glibenclamide (Glb), Ac-YVAD-CHO, and KCl for 3 h <i>(A)</i> and 18 h <i>(B&C)</i>. Macrophages incubated with media alone were used as controls. <i>(A&B)</i> IL-1β release in supernatants was determined by an ELISA. <i>(C)</i> Quantitative PCR analysis of parasite burden in infected macrophages using <i>Tc18SrDNA</i>-specific oligonucleotides.