Analytical gel filtration of StpA and the StpA<sub>M4T</sub>, StpA<sub>A77D</sub>, and StpA<sub>T37I</sub> variants.
- Sabrina S. Ali (655283)
- Jeremy Soo (655284)
- Chitong Rao (584124)
- Andrea S. Leung (655285)
- David Hon-Man Ngai (655286)
- Alexander W. Ensminger (160791)
- William Wiley Navarre (619672)
Publication date
November 2014
DOIAbstract
<p>Purified StpA<sub>WT</sub>, StpA<sub>T37I</sub>, StpA<sub>M4T</sub>, StpA<sub>A77D</sub> proteins at a concentration of 100 µM were applied to a Superdex 200 10/300 GL column pre-equilibrated with 20 mM Tris pH 8, 1 M NaCl, 1 mM EDTA and 5% glycerol. The column was calibrated using globular protein standards ranging from 12.4 kDa to 443 kDa.</p
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