<i>P. gingivalis</i> biofilm development.

<p>Orthogonal projections of CLSM images showing a representative region of the x-y plane over the depth of the biofilm in both xz and yz dimensions of the ATCC 33277 wild-type, <i>har</i> mutant ECR455 and <i>har</i> complement ECR 475 strains grown in excess hemin (<b>A</b>) or hemin-limitation (<b>B</b>). Comparison of the Biovolume (<b>C</b>), Average Thickness (<b>D</b>) and SA:Biovolume (<b>E</b>) calculated for each strain's biofilm growth in either excess hemin (dark bars) or limited hemin (light bars) over three independent experiments. All biometric parameters analysed for the biofilms formed by ATCC 33277 and ECR475 were significantly (p<0.005) altered when hemin was limited. * indicates a significant difference in excess hemin versus limited hemin (p<0.005); ** indicates a significant difference in ECR455 biovolume, average thickness and SA:biovolume when compared to the same biometric parameters of both the ATCC 33277 and ECR475 biofilms (p<0.001).