Dose and time related acute ear inflammation changes in response to topical CPAF.

<p><i>1A</i>. <i>Topical CPAF dose-dependently induces rapid inflammatory responses as measured by ear thickness measurements.</i> One ear of WT and <i>Ptafr</i> (-/-) were treated with one of three doses of CPAF (20 µl of a 0.1, 0.3, and 1.0 mM solution for a total treatment dose of 2, 6, or 20 nmole CPAF per ear). The contralateral ear was treated with acetone alone (VEH). Ear thickness was measured prior to treatment and 2 hours after treatment. After the pretreatment ear thickness values were subtracted, the mean and SEM were plotted (n = 4 for 20 nmole and n = 8 for 2 & 6 nmole CPAF & VEH treated mouse ears). <i>1B</i>. <i>Topical CPAF treatment induces a rapid, but transient increase in inflammation as measured by ear thickness changes.</i> One ear of wildtype (WT) and <i>Ptafr</i> (-/-) mice was treated with 20 µl of CPAF (20 nmoles of a 0.1 mM solution in acetone) and 20 µl of acetone (VEH) was applied to the contralateral ear. Ear thickness was measured just prior to reagent application and at 1, 2, 4, and 8 hours after application. Results represent the mean and SEM (n = 4 mice) after subtracting the pretreatment ear thickness. CPAF induced a significant increase in ear thickness in WT mice relative to the WT+VEH treated ears. *, <i>p</i><0.05; **, <i>p</i><0.01; ***; <i>p</i><0.001; 2-tailed <i>t</i>-test.