The density of proliferating cells in the Main Proliferative Zone (MPZ).

<p>A–D. Volume number density of proliferating cells in the MPZ (A), the rostral migratory stream (RMS)(B), the caudate migratory stream (CMS) (C), the midlayer (D). Each EdU-labeled nucleus is shown as a colored dot with colors assigned according to the number of neighboring EdU-labeled nuclei located closer than 200 µm to this nucleus. Scale is shown on the right. E–F. Average number of neighboring EdU-labeled nuclei located closer than 200 µm to each EdU-labeled nucleus from main categories of proliferating cells in the adult mouse brain (E) or different parts of the caudate migratory stream (F). Abbreviated names are given according to <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0111453#pone-0111453-t002" target="_blank">Table 2</a>. G, I, K. Density of proliferating cells in the RMS (G), CMS (I) and midlayer (K) along the anteroposterior axis of the brain. The average number of neighboring EdU-labeled nuclei located closer than 200 µm to each EdU-labeled nucleus in a 50 µm brain section is shown along the vertical axis of the charts. The distance from the anterior end of the brain is shown along the horizontal axis of the charts. H, J, L. Distribution of proliferating cells in the RMS (G), CMS (I) and midlayer (K) along the anteroposterior axis of the brain. The number of EdU-labeled nuclei in a 50 µm brain section is shown along the vertical axis of the charts. The distance from the anterior end of the brain is shown along the horizontal axis of the charts. K, L. The blue line shows the density (K) and distribution (L) of all proliferating cells located in the midlayer. The red line shows the density (K) and distribution (L) of proliferating cells located in the midlayer, with the exception of 250 cells located close to the CMS (these cells are shown with black arrow on panel D). 120 days-old mouse one hour post EdU injection.